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验证数据展示

  • IHC staining of mouse brain using Biotin-15044

    IHC staining of mouse brain using Biotin-15044

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-15044 (GPM6A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Biotin Plus-conjugated GPM6A Polyclonal antibody

GPM6A Polyclonal Antibody for IHC
Cat No. Biotin-15044

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

Neuronal membrane glycoprotein M6-a, M6A, GPM6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin Plus
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-15044 targets GPM6A in IHC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC Application Description
Tested Reactivity human, mouse, rat
Immunogen GPM6A fusion protein Ag6915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycoprotein M6A
Synonyms Neuronal membrane glycoprotein M6-a, M6A, GPM6
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC022528
Gene Symbol GPM6A
Gene ID (NCBI) 2823
Conjugate Biotin Plus
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51674
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycoprotein M6A (GPM6A, or M6A) is known as a multi-pass transmembrane protein and an abundant cell surface protein on neurons in the central nervous system (CNS). M6A belongs to the myelin proteolipid protein family and is involved in neuronal differentiation, including differentiation and migration of neuronal stem cell. M6A is identified as a retinal membrane protein that is strongly expressed at embryonic stages. In mouse adult retina, M6A plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation via regulation of MAPK and Src signaling pathways.

实验方案

Product Specific Protocols
IHC protocol for Biotin Plus GPM6A antibody Biotin-15044Download protocol
Standard Protocols
Click here to view our Standard Protocols