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验证数据展示

  • WB analysis of THP-1 using 84160-3-RR (same clone as 84160-3-PBS)

    WB analysis of THP-1 using 84160-3-RR (same clone as 84160-3-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84160-3-RR (same clone as 84160-3-PBS)

    IF Staining of HeLa using 84160-3-RR (same clone as 84160-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (84160-3-RR, Clone: 241435F3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01096-1

    Sandwich ELISA standard curve of MP01096-1

    Sandwich ELISA standard curve of MP01096-1, Human GPNMB Recombinant Matched Antibody Pair - PBS only. 84160-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14124. 84160-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Sample test of MP01096-1

    Sample test of MP01096-1

    Plasma of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 12.18 ng/mL with a range of 3.43-30.61 ng/mL.

  • Sample test of MP01096-1

    Sample test of MP01096-1

    Serum of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 5.69 ng/mL with a range of 3.96-8.17 ng/mL.

  • Cytometric bead array standard curve of MP01096-1

    Cytometric bead array standard curve of MP01096-1

    Cytometric bead array standard curve of MP01096-1, GPNMB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84160-3-PBS. Detection antibody: 84160-2-PBS. Standard: Ag14124. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84160-3-RR

    Affinity and Kinetic Characterization of 84160-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84160-3-RR against Human GPNMB were performed. The affinity constant is below 1 pM.

GPNMB Recombinant antibody, PBS Only (Capture)

GPNMB Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84160-3-PBS

产品说明书

CloneNo. 241435F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GPNMB Ready-To-Use IHC Kit. GPNMB primary antibody included.

产品信息

84160-3-PBS targets GPNMB as part of a matched antibody pair:

MP01096-1: 84160-3-PBS capture and 84160-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 GPNMB fusion protein Ag14124 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycoprotein (transmembrane) nmb
别名 UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type
计算分子量 64 kDa
观测分子量120 kDa, 95 kDa
GenBank蛋白编号BC011595
基因名称 GPNMB
Gene ID (NCBI) 10457
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14956
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.