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验证数据展示

  • WB analysis of THP-1 using 84160-3-RR

    WB analysis of THP-1 using 84160-3-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84160-3-RR

    IF Staining of HeLa using 84160-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (84160-3-RR, Clone: 241435F3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84160-3-RR

    Affinity and Kinetic Characterization of 84160-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84160-3-RR against Human GPNMB were performed. The affinity constant is below 1 pM.

GPNMB Recombinant antibody

GPNMB Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84160-3-RR

Print datasheet

CloneNo. 241435F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, A375 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GPNMB Ready-To-Use IHC Kit. GPNMB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84160-3-RR targets GPNMB in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen GPNMB fusion protein Ag14124 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycoprotein (transmembrane) nmb
Synonyms UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type
Calculated Molecular Weight 64 kDa
Observed Molecular Weight120 kDa, 95 kDa
GenBank Accession NumberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
RRIDAB_3671722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

实验方案

Product Specific Protocols
WB protocol for GPNMB antibody 84160-3-RRDownload protocol
IF protocol for GPNMB antibody 84160-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols