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验证数据展示

  • IF Staining of HeLa using CL488-66926

    IF Staining of HeLa using CL488-66926

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 GPNMB antibody (CL488-66926, Clone: 2B10B8 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated GPNMB Monoclonal antibody

GPNMB Monoclonal Antibody for IF/ICC
Cat No. CL488-66926

Print datasheet

CloneNo. 2B10B8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GPNMB Ready-To-Use IHC Kit. GPNMB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66926-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66926 targets GPNMB in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen GPNMB fusion protein Ag26747 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycoprotein (transmembrane) nmb
Synonyms GPNMB, HGFIN, Transmembrane glycoprotein NMB
Calculated Molecular Weight 64 kDa
GenBank Accession NumberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
RRIDAB_2919390
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14956
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GPNMB antibody CL488-66926Download protocol
Standard Protocols
Click here to view our Standard Protocols