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GPR105 Recombinant antibody

GPR105 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240498B10

Cat no : 83511-1-RR

Print datasheet

Synonyms

P2RY14, P2Y purinoceptor 14, KIAA0001, G-protein coupled receptor 105, G protein coupled receptor 105

验证数据展示

  • WB analysis using 83511-1-RR

    WB analysis using 83511-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83511-1-RR (GPR105 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83511-1-RR

    Affinity and Kinetic Characterization of 83511-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83511-1-RR against Human GPR105 were performed. The affinity constant is 3.59 nM.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells, human placenta tissue, mouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83511-1-RR targets GPR105 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GPR105 fusion protein Ag14105 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name purinergic receptor P2Y, G-protein coupled, 14
Synonyms P2RY14, P2Y purinoceptor 14, KIAA0001, G-protein coupled receptor 105, G protein coupled receptor 105
Calculated Molecular Weight 338 aa, 39 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC034989
Gene Symbol P2RY14
Gene ID (NCBI) 9934
RRIDAB_3671136
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15391
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR105 (P2RY14) is widely expressed throughout many brain regions and peripheral tissues of humans and rodents, and couples to a pertussis toxin-sensitive G protein (PMID: 14559350). GPR105 is also prominently expressed in immune cells, including macrophages, lymphocytes, and neutrophils (PMID: 22778393).

实验方案

Product Specific Protocols
WB protocol for GPR105 antibody 83511-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols