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GPR105 Recombinant antibody

GPR105 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240498B10

Cat no : 83511-1-RR

Print datasheet

Synonyms

P2RY14, P2Y purinoceptor 14, KIAA0001, G-protein coupled receptor 105, G protein coupled receptor 105

验证数据展示

  • WB analysis using 83511-1-RR

    WB analysis using 83511-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83511-1-RR (GPR105 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83511-1-RR

    Affinity and Kinetic Characterization of 83511-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83511-1-RR against Human GPR105 were performed. The affinity constant is 3.59 nM.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells, human placenta tissue, mouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83511-1-RR is GPR105 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GPR105 fusion protein Ag14105 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name purinergic receptor P2Y, G-protein coupled, 14
Synonyms P2RY14, P2Y purinoceptor 14, KIAA0001, G-protein coupled receptor 105, G protein coupled receptor 105
Calculated Molecular Weight 338 aa, 39 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC034989
Gene Symbol P2RY14
Gene ID (NCBI) 9934
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15391
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR105 (P2RY14) is widely expressed throughout many brain regions and peripheral tissues of humans and rodents, and couples to a pertussis toxin-sensitive G protein (PMID: 14559350). GPR105 is also prominently expressed in immune cells, including macrophages, lymphocytes, and neutrophils (PMID: 22778393).

实验方案

Product Specific Protocols
WB protocol for GPR105 antibody 83511-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols