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验证数据展示

  • IF Staining of ARPE-19 using 29328-1-AP

    IF Staining of ARPE-19 using 29328-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • WB analysis using 29328-1-AP

    WB analysis using 29328-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29328-1-AP (GPR161 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GPR161 Polyclonal antibody

GPR161 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29328-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

FLJ33952, G protein coupled receptor 161, G protein coupled receptor RE2, GPR161, RE2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inARPE-19 cells, hTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29328-1-AP targets GPR161 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 GPR161 fusion protein Ag30998 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 161
别名 FLJ33952, G protein coupled receptor 161, G protein coupled receptor RE2, GPR161, RE2
计算分子量 529 aa, 59 kDa
观测分子量70 kDa
GenBank蛋白编号BC028163
基因名称 GPR161
Gene ID (NCBI) 23432
RRIDAB_3086118
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6U8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band detected by this antibody probably represents a modified version of GPR161 (PMID: 18250320).

实验方案

Product Specific Protocols
WB protocol for GPR161 antibody 29328-1-APDownload protocol
IF protocol for GPR161 antibody 29328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols