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GPR161 Polyclonal antibody

GPR161 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29328-1-AP

Print datasheet

Synonyms

FLJ33952, G protein coupled receptor 161, G protein coupled receptor RE2, GPR161, RE2

验证数据展示

  • IF Staining of ARPE-19 using 29328-1-AP

    IF Staining of ARPE-19 using 29328-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed ARPE-19 cells using GPR161 antibody (29328-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • WB analysis using 29328-1-AP

    WB analysis using 29328-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29328-1-AP (GPR161 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inARPE-19 cells, hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29328-1-AP targets GPR161 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GPR161 fusion protein Ag30998 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 161
Synonyms FLJ33952, G protein coupled receptor 161, G protein coupled receptor RE2, GPR161, RE2
Calculated Molecular Weight 529 aa, 59 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC028163
Gene Symbol GPR161
Gene ID (NCBI) 23432
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6U8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band detected by this antibody probably represents a modified version of GPR161 (PMID: 18250320).

实验方案

Product Specific Protocols
WB protocol for GPR161 antibody 29328-1-APDownload protocol
IF protocol for GPR161 antibody 29328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols