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验证数据展示

  • FC experiment of A549 using CL647-55248

    FC experiment of A549 using CL647-55248

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human GPR35 (CL647-55248) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated GPR35 Polyclonal antibody

GPR35 Polyclonal Antibody for FC (Intra)
Cat No. CL647-55248

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

Kynurenic acid receptor, KYNA receptor, G-protein coupled receptor 35, G protein coupled receptor 35

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 55248-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-55248 targets GPR35 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 35
别名 Kynurenic acid receptor, KYNA receptor, G-protein coupled receptor 35, G protein coupled receptor 35
计算分子量 34 kDa
GenBank蛋白编号NM_005301
基因名称 GPR35
Gene ID (NCBI) 2859
RRIDAB_2883650
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HC97
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR35, also named as KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 GPR35 antibody CL647-55248Download protocol
Standard Protocols
Click here to view our Standard Protocols