验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | SH-SY5Y cells, mouse Hippocamus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
21514-1-AP targets GPR50 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | GPR50 fusion protein Ag16040 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | G protein-coupled receptor 50 |
| 别名 | Melatonin related receptor, Mel1c, H9, G protein-coupled receptor 50, G protein coupled receptor 50 |
| 计算分子量 | 617 aa, 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC103696 |
| 基因名称 | GPR50 |
| Gene ID (NCBI) | 9248 |
| RRID | AB_2878874 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13585 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR50 antibody 21514-1-AP | Download protocol |
| IP protocol for GPR50 antibody 21514-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |