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CSN1 Polyclonal antibody

CSN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat No : 11709-1-AP

Print datasheet

Synonyms

GPS1, SGN1, JAB1-containing signalosome subunit 1, GPS-1, COPS1

验证数据展示

  • IP experiment of HeLa using 11709-1-AP

    IP experiment of HeLa using 11709-1-AP

    IP result of anti-CSN1 (IP:11709-1-AP, 4ug; Detection:11709-1-AP 1:500) with HeLa cells lysate 2600 ug.

  • IHC staining of human prostate cancer using 11709-1-AP

    IHC staining of human prostate cancer using 11709-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11709-1-AP (CSN1 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of MCF-7 using 11709-1-AP

    IF Staining of MCF-7 using 11709-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11709-1-AP (CSN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 11709-1-AP

    WB analysis using 11709-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HT-1080 cells, Raji cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11709-1-AP targets CSN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CSN1 fusion protein Ag2273 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein pathway suppressor 1
Synonyms GPS1, SGN1, JAB1-containing signalosome subunit 1, GPS-1, COPS1
Calculated Molecular Weight 491 aa, 55-60 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC000155
Gene Symbol CSN1
Gene ID (NCBI) 2873
RRIDAB_2232685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSN1 antibody 11709-1-APDownload protocol
IHC protocol for CSN1 antibody 11709-1-APDownload protocol
IF protocol for CSN1 antibody 11709-1-APDownload protocol
IP protocol for CSN1 antibody 11709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Med Rep

CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.

Authors - Hangwei Fu
View Article