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验证数据展示

  • IP experiment of HeLa using 11709-1-AP

    IP experiment of HeLa using 11709-1-AP

    IP result of anti-CSN1 (IP:11709-1-AP, 4ug; Detection:11709-1-AP 1:500) with HeLa cells lysate 2600 ug.

  • IHC staining of human prostate cancer using 11709-1-AP

    IHC staining of human prostate cancer using 11709-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11709-1-AP (CSN1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with GPS1/CSN1 antibody included
  • IF Staining of MCF-7 using 11709-1-AP

    IF Staining of MCF-7 using 11709-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11709-1-AP (CSN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 11709-1-AP

    WB analysis using 11709-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11709-1-AP (CSN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CSN1 Polyclonal antibody

CSN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11709-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

GPS1, SGN1, JAB1-containing signalosome subunit 1, GPS-1, COPS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HT-1080 cells, Raji cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GPS1/CSN1 Ready-To-Use IHC Kit. GPS1/CSN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11709-1-AP targets CSN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 CSN1 fusion protein Ag2273 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein pathway suppressor 1
别名 GPS1, SGN1, JAB1-containing signalosome subunit 1, GPS-1, COPS1
计算分子量 491 aa, 55-60 kDa
观测分子量 55-70 kDa
GenBank蛋白编号BC000155
基因名称 CSN1
Gene ID (NCBI) 2873
RRIDAB_2232685
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13098
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSN1 antibody 11709-1-APDownload protocol
IHC protocol for CSN1 antibody 11709-1-APDownload protocol
IF protocol for CSN1 antibody 11709-1-APDownload protocol
IP protocol for CSN1 antibody 11709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Med Rep

CSN1 facilitates proliferation and migration of hepatocellular carcinoma cells by upregulating cyclin A2 expression.

Authors - Hangwei Fu
View Article