验证数据展示
经过测试的应用
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82949-1-RR targets GPX4 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | GPX4 fusion protein Ag5809 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | glutathione peroxidase 4 (phospholipid hydroperoxidase) |
Synonyms | GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12 |
Calculated Molecular Weight | 22 kDa |
GenBank Accession Number | BC022071 |
Gene Symbol | GPX4 |
Gene ID (NCBI) | 2879 |
RRID | AB_3670692 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P36969 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.
实验方案
Product Specific Protocols | |
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IHC protocol for GPX4 antibody 82949-1-RR | Download protocol |
IF protocol for GPX4 antibody 82949-1-RR | Download protocol |
FC protocol for GPX4 antibody 82949-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |