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验证数据展示

  • IF Staining of HepG2 using 82949-1-RR

    IF Staining of HepG2 using 82949-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GPX4 antibody (82949-1-RR, Clone: 230168A8 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 82949-1-RR

    FC experiment of HeLa using 82949-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GPX4 Recombinant antibody (82949-1-RR, Clone:230168A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse testis using 82949-1-RR

    IHC staining of mouse testis using 82949-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82949-1-RR (GPX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 82949-1-RR

    IHC staining of mouse testis using 82949-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82949-1-RR (GPX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82949-1-RR

    Affinity and Kinetic Characterization of 82949-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82949-1-RR against Human GCLM were performed. The affinity constant is 1 pM.

GPX4 Recombinant antibody

GPX4 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82949-1-RR

产品说明书

CloneNo. 230168A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82949-1-RR targets GPX4 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 GPX4 fusion protein Ag5809 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glutathione peroxidase 4 (phospholipid hydroperoxidase)
别名 GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12
计算分子量 22 kDa
GenBank蛋白编号BC022071
基因名称 GPX4
Gene ID (NCBI) 2879
RRIDAB_3670692
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36969
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

实验方案

Product Specific Protocols
IHC protocol for GPX4 antibody 82949-1-RRDownload protocol
IF protocol for GPX4 antibody 82949-1-RRDownload protocol
FC protocol for GPX4 antibody 82949-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols