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CoraLite® Plus 488-conjugated GPX4 Recombinant antibody

GPX4 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230168A8

Cat No : CL488-82949

Print datasheet

Synonyms

GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

验证数据展示

  • IF Staining of HepG2 using CL488-82949

    IF Staining of HepG2 using CL488-82949

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GPX4 antibody (CL488-82949, Clone: 230168A8 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 82949-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82949 targets GPX4 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen GPX4 fusion protein Ag5809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms GSHPx 4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12
Calculated Molecular Weight 22 kDa
GenBank Accession NumberBC022071
Gene Symbol GPX4
Gene ID (NCBI) 2879
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GPX4 antibody CL488-82949Download protocol
Standard Protocols
Click here to view our Standard Protocols