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  • Featured Product
  • KD/KO Validated

GPX4 (Human Specific) Recombinant antibody, PBS Only

GPX4 (Human Specific) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E9

Cat no : 82822-2-PBS

Print datasheet

Synonyms

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

验证数据展示

  • WB analysis using 82822-2-RR (same clone as 82822-2-PBS)

    WB analysis using 82822-2-RR (same clone as 82822-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82822-2-RR (same clone as 82822-2-PBS)

    WB analysis of HEK-293 using 82822-2-RR (same clone as 82822-2-PBS)

    WB result of GPX4 (Human Specific) antibody (82822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 (Human Specific) transfected HEK-293 cells. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82822-2-RR

    Affinity and Kinetic Characterization of 82822-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

产品信息

82822-2-PBS targets GPX4 (Human Specific) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GPX4 (Human Specific) fusion protein Ag30650 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.