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GPX4 (Human Specific) Recombinant antibody, PBS Only

GPX4 (Human Specific) Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E9

Cat No : 82822-2-PBS

Print datasheet

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx

验证数据展示

  • WB analysis using 82822-2-RR (same clone as 82822-2-PBS)

    WB analysis using 82822-2-RR (same clone as 82822-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 82822-2-RR (same clone as 82822-2-PBS)

    IF Staining of mouse testis using 82822-2-RR (same clone as 82822-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using GPX4 (Human Specific) antibody (82822-2-RR, Clone: 6E9 ) at dilution of 1:750 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

产品信息

82822-2-PBS targets GPX4 (Human Specific) in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen GPX4 (Human Specific) fusion protein Ag30650 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.