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GPX4 (Human Specific) Recombinant antibody

GPX4 (Human Specific) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

6E9

Cat no : 82822-2-RR

Print datasheet

Synonyms

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

验证数据展示

  • WB analysis using 82822-2-RR

    WB analysis using 82822-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82822-2-RR

    WB analysis of HEK-293 using 82822-2-RR

    WB result of GPX4 (Human Specific) antibody (82822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 (Human Specific) transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82822-2-RR

    Affinity and Kinetic Characterization of 82822-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:15000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

82822-2-RR targets GPX4 (Human Specific) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GPX4 (Human Specific) fusion protein Ag30650 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.

实验方案

Product Specific Protocols
WB protocol for GPX4 (Human Specific) antibody 82822-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

HDAC inhibitors activate lipid peroxidation and ferroptosis in gastric cancer

Authors - Robert Jenke
View Article