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  • Featured Product
  • KD/KO Validated

GRAMD1B Polyclonal antibody

GRAMD1B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24905-1-AP

Print datasheet

Synonyms

UNQ3032/PRO9834, Protein Aster-B, KIAA1201, GRAM domain-containing protein 1B, GRAM domain containing 1B

验证数据展示

  • WB analysis of A549 using 24905-1-AP

    WB analysis of A549 using 24905-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24905-1-AP (GRAMD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24905-1-AP

    IHC staining of human liver cancer using 24905-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 24905-1-AP

    IHC staining of human liver cancer using 24905-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 24905-1-AP

    IF Staining of A549 using 24905-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 24905-1-AP (GRAMD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of A549 using 24905-1-AP

    FC experiment of A549 using 24905-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human GRAMD1B (24905-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24905-1-AP targets GRAMD1B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen GRAMD1B fusion protein Ag20670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GRAM domain containing 1B
Synonyms UNQ3032/PRO9834, Protein Aster-B, KIAA1201, GRAM domain-containing protein 1B, GRAM domain containing 1B
Calculated Molecular Weight 738 aa, 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC107480
Gene Symbol GRAMD1B
Gene ID (NCBI) 57476
RRIDAB_2879791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KR37
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.

实验方案

Product Specific Protocols
WB protocol for GRAMD1B antibody 24905-1-APDownload protocol
IHC protocol for GRAMD1B antibody 24905-1-APDownload protocol
IF protocol for GRAMD1B antibody 24905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Metab

Aster-B coordinates with Arf1 to regulate mitochondrial cholesterol transport.

Authors - John-Paul Andersen
View Article
humanWB,IF

J Lipid Res

ASTER-B regulates mitochondrial carotenoid transport and homeostasis

Authors - Sepalika Bandara
  • KD Validated
View Article
humanWB

Noncoding RNA

MiR-4646-5p Acts as a Tumor-Suppressive Factor in Triple Negative Breast Cancer and Targets the Cholesterol Transport Protein GRAMD1B

Authors - Katharina Jonas
View Article
humanWB,IF

Exp Eye Res

Imaging Macular Carotenoids and Their Related Proteins in the Human Retina with Confocal Resonance Raman and Fluorescence Microscopy

Authors - Binxing Li
View Article
humanWB,IF

bioRxiv

Intracellular Retention of Estradiol is Augmented by GRAM Domain Containing Protein ASTER-B in Breast Cancer Cells

Authors - Hyung Bum Kim
  • KD Validated
View Article