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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GRAMD1B Polyclonal antibody

GRAMD1B Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24905

Print datasheet

Synonyms

UNQ3032/PRO9834, Protein Aster-B, KIAA1201, GRAM domain-containing protein 1B, GRAM domain containing 1B

验证数据展示

  • IF Staining of A549 using CL488-24905

    IF Staining of A549 using CL488-24905

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 GRAMD1B antibody (CL488-24905) at dilution of 1:200.

  • FC experiment of A549 using CL488-24905

    FC experiment of A549 using CL488-24905

    1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Gramd1b Polyclonal Antibody (CL488-24905)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 24905-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24905 targets GRAMD1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen GRAMD1B fusion protein Ag20670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GRAM domain containing 1B
Synonyms UNQ3032/PRO9834, Protein Aster-B, KIAA1201, GRAM domain-containing protein 1B, GRAM domain containing 1B
Calculated Molecular Weight 738 aa, 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC107480
Gene Symbol GRAMD1B
Gene ID (NCBI) 57476
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KR37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GRAMD1B antibody CL488-24905Download protocol
FC protocol for CL Plus 488 GRAMD1B antibody CL488-24905Download protocol
Standard Protocols
Click here to view our Standard Protocols