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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GRAMD1B Polyclonal antibody

GRAMD1B Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24905

Print datasheet

Synonyms

UNQ3032/PRO9834, Protein Aster-B, KIAA1201, GRAM domain-containing protein 1B, GRAM domain containing 1B

验证数据展示

  • IF Staining of A549 using CL488-24905

    IF Staining of A549 using CL488-24905

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 GRAMD1B antibody (CL488-24905) at dilution of 1:200.

  • FC experiment of A549 using CL488-24905

    FC experiment of A549 using CL488-24905

    1x10^6 A549 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Gramd1b Polyclonal Antibody (CL488-24905)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 24905-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24905 targets GRAMD1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen GRAMD1B fusion protein Ag20670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GRAM domain containing 1B
Synonyms UNQ3032/PRO9834, Protein Aster-B, KIAA1201, GRAM domain-containing protein 1B, GRAM domain containing 1B
Calculated Molecular Weight 738 aa, 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC107480
Gene Symbol GRAMD1B
Gene ID (NCBI) 57476
RRIDAB_3672780
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KR37
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GRAMD1B antibody CL488-24905Download protocol
FC protocol for CL Plus 488 GRAMD1B antibody CL488-24905Download protocol
Standard Protocols
Click here to view our Standard Protocols