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验证数据展示

  • WB analysis of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    WB analysis of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 24905-1-AP (GRAMD1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 24905-1-AP (same clone as 24905-1-PBS)

    IHC staining of human liver cancer using 24905-1-AP (same clone as 24905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 24905-1-AP (same clone as 24905-1-PBS)

    IHC staining of human liver cancer using 24905-1-AP (same clone as 24905-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24905-1-AP (GRAMD1B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    IF Staining of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 24905-1-AP (GRAMD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    FC experiment of A549 using 24905-1-AP (same clone as 24905-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human GRAMD1B (24905-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24905-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GRAMD1B Polyclonal antibody, PBS Only

GRAMD1B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 24905-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

24905-1-PBS targets GRAMD1B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 GRAMD1B fusion protein Ag20670 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRAM domain containing 1B
别名
计算分子量 738 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC107480
基因名称 GRAMD1B
Gene ID (NCBI) 57476
RRIDAB_2879791
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KR37
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.