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GRP75 Monoclonal antibody, PBS Only

GRP75 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B12F2

Cat no : 67563-1-PBS

Print datasheet

Synonyms

CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74

验证数据展示

  • WB analysis using 67563-1-Ig (same clone as 67563-1-PBS)

    WB analysis using 67563-1-Ig (same clone as 67563-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67563-1-Ig (GRP75 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

    IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA9/GRP75 antibody included
  • IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

    IHC staining of human breast cancer using 67563-1-Ig (same clone as 67563-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67563-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSPA9/GRP75 antibody included
Planning an IHC experiment? We recommend our IHCeasy HSPA9/GRP75 Ready-To-Use IHC Kit. HSPA9/GRP75 primary antibody included.

产品信息

67563-1-PBS targets GRP75 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GRP75 fusion protein Ag7125 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 9 (mortalin)
Synonyms CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC000478
Gene Symbol GRP75
Gene ID (NCBI) 3313
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38646
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)