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GRP75 Monoclonal antibody

GRP75 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12F2

Cat no : 67563-1-Ig

Print datasheet

Synonyms

CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74

验证数据展示

  • WB analysis using 67563-1-Ig

    WB analysis using 67563-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67563-1-Ig (GRP75 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67563-1-Ig

    IHC staining of human breast cancer using 67563-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPA9/GRP75 antibody included
  • IHC staining of human breast cancer using 67563-1-Ig

    IHC staining of human breast cancer using 67563-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67563-1-Ig (GRP75 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPA9/GRP75 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSPA9/GRP75 Ready-To-Use IHC Kit. HSPA9/GRP75 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67563-1-Ig targets GRP75 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GRP75 fusion protein Ag7125 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 9 (mortalin)
Synonyms CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC000478
Gene Symbol GRP75
Gene ID (NCBI) 3313
RRIDAB_2882777
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38646
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 229209024)

实验方案

Product Specific Protocols
WB protocol for GRP75 antibody 67563-1-IgDownload protocol
IHC protocol for GRP75 antibody 67563-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols