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验证数据展示

  • IF Staining of HepG2 using CL594-67563

    IF Staining of HepG2 using CL594-67563

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated GRP75 antibody (CL594-67563, Clone: 2B12F2 ) at dilution of 1:100.

CoraLite®594-conjugated GRP75 Monoclonal antibody

GRP75 Monoclonal Antibody for IF/ICC
Cat No. CL594-67563

产品说明书

CloneNo. 2B12F2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPA9/GRP75 Ready-To-Use IHC Kit. HSPA9/GRP75 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67563-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67563 targets GRP75 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 GRP75 fusion protein Ag7125 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 70kDa protein 9 (mortalin)
别名 CSA, GRP 75, GRP75, Heat shock 70 kDa protein 9, HSPA9, HSPA9B, Mortalin, MOT, mot 2, MOT2, MTHSP75, PBP74, Peptide binding protein 74
计算分子量 74 kDa
观测分子量 75 kDa
GenBank蛋白编号BC000478
基因名称 GRP75
Gene ID (NCBI) 3313
RRIDAB_2920160
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP38646
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. (PMID: 21640711, 22920904)

实验方案

Product Specific Protocols
IF protocol for CL594 GRP75 antibody CL594-67563Download protocol
Standard Protocols
Click here to view our Standard Protocols