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GRP94 Recombinant antibody, PBS Only

GRP94 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230502B7

Cat no : 83147-5-PBS

Print datasheet

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

验证数据展示

  • WB analysis using 83147-5-RR (same clone as 83147-5-PBS)

    WB analysis using 83147-5-RR (same clone as 83147-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83147-5-RR (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83147-5-RR (same clone as 83147-5-PBS)

    FC experiment of A431 using 83147-5-RR (same clone as 83147-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Grp94 Recombinant Antibody (83147-5-RR, Clone:230502B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IP experiment of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IP result of anti-GRP94 (IP:83147-5-RR, 4ug; Detection:83147-5-RR 1:5000) with HeLa cells lysate 1260 ug. This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 83147-5-RR (same clone as 83147-5-PBS)

    IF Staining of HEK-293 using 83147-5-RR (same clone as 83147-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83147-5-RR

    Affinity and Kinetic Characterization of 83147-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83147-5-RR against Human GRP94 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

产品信息

83147-5-PBS targets GRP94 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, pig
Immunogen GRP94 fusion protein Ag1439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock protein 90kDa beta (Grp94), member 1
Synonyms HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC009195
Gene Symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP14625
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).