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GRP94 Recombinant antibody, PBS Only

GRP94 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

pig, human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230502B7

Cat no : 83147-5-PBS

Print datasheet

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

验证数据展示

  • WB analysis using 83147-5-RR (same clone as 83147-5-PBS)

    WB analysis using 83147-5-RR (same clone as 83147-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83147-5-RR (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    IHC staining of human lung cancer using 83147-5-RR (same clone as 83147-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IF Staining of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83147-5-RR (same clone as 83147-5-PBS)

    FC experiment of A431 using 83147-5-RR (same clone as 83147-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Grp94 Recombinant Antibody (83147-5-RR, Clone:230502B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IP experiment of HeLa using 83147-5-RR (same clone as 83147-5-PBS)

    IP result of anti-GRP94 (IP:83147-5-RR, 4ug; Detection:83147-5-RR 1:5000) with HeLa cells lysate 1260 ug. This data was developed using the same antibody clone with 83147-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83147-5-RR

    Affinity and Kinetic Characterization of 83147-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83147-5-RR against Human GRP94 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

产品信息

83147-5-PBS targets GRP94 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with pig, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity pig, human
Immunogen GRP94 fusion protein Ag1439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock protein 90kDa beta (Grp94), member 1
Synonyms HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP
Calculated Molecular Weight 96 kDa
GenBank Accession NumberBC009195
Gene Symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP14625
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C