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GRP94 Recombinant antibody

GRP94 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

230502B7

Cat no : 83147-5-RR

Print datasheet

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

验证数据展示

  • WB analysis using 83147-5-RR

    WB analysis using 83147-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83147-5-RR (GRP94 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 83147-5-RR

    IHC staining of human lung cancer using 83147-5-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IHC staining of human lung cancer using 83147-5-RR

    IHC staining of human lung cancer using 83147-5-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83147-5-RR (GRP94 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRP94/HSP90B1 antibody included
  • IF Staining of HeLa using 83147-5-RR

    IF Staining of HeLa using 83147-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of HeLa using 83147-5-RR

    IF Staining of HeLa using 83147-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GRP94 antibody (83147-5-RR, Clone: 230502B7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 83147-5-RR

    FC experiment of A431 using 83147-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Grp94 Recombinant Antibody (83147-5-RR, Clone:230502B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of HeLa using 83147-5-RR

    IP experiment of HeLa using 83147-5-RR

    IP result of anti-GRP94 (IP:83147-5-RR, 4ug; Detection:83147-5-RR 1:5000) with HeLa cells lysate 1260 ug.

  • Affinity and Kinetic Characterization of 83147-5-RR

    Affinity and Kinetic Characterization of 83147-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83147-5-RR against Human GRP94 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, LNCaP cells, pig brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83147-5-RR targets GRP94 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, pig
Immunogen GRP94 fusion protein Ag1439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock protein 90kDa beta (Grp94), member 1
Synonyms HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC009195
Gene Symbol GRP94
Gene ID (NCBI) 7184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP14625
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

实验方案

Product Specific Protocols
WB protocol for GRP94 antibody 83147-5-RRDownload protocol
IHC protocol for GRP94 antibody 83147-5-RRDownload protocol
IF protocol for GRP94 antibody 83147-5-RRDownload protocol
IP protocol for GRP94 antibody 83147-5-RRDownload protocol
FC protocol for GRP94 antibody 83147-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols