CoraLite® Plus 750-conjugated GRP94 Recombinant antibody
GRP94 Uni-rAbTM Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
6G15
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GRP94/HSP90B1 Ready-To-Use IHC Kit. GRP94/HSP90B1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82805-2-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-82805-2 targets GRP94 in WB applications and shows reactivity with human samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | human |
| Immunogen | GRP94 fusion protein Ag6344 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Synonyms | HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP |
| Calculated Molecular Weight | 92 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC066656 |
| Gene Symbol | GRP94 |
| Gene ID (NCBI) | 7184 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14625 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 GRP94 antibody CL750-82805-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |