验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A431 cells, COLO 320 cells, EC109 cells, HL-60 cells, human colon tissue, human small intestine tissue, K-562 cells, Raji cells, TF-1 cells, PC-3 cells, U2OS cells, A549 cells, HepG2 cells, Jurkat cells |
Positive IP detected in | THP-1 cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66387-1-Ig targets GSDMD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat |
免疫原 | GSDMD fusion protein Ag16827 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | gasdermin D |
别名 | DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1 |
计算分子量 | 484 aa, 53 kDa |
观测分子量 | 47-53 kDa |
GenBank蛋白编号 | BC008904 |
基因名称 | GSDMD |
Gene ID (NCBI) | 79792 |
RRID | AB_2881763 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P57764 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
实验方案
Product Specific Protocols | |
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WB protocol for GSDMD antibody 66387-1-Ig | Download protocol |
IF protocol for GSDMD antibody 66387-1-Ig | Download protocol |
IP protocol for GSDMD antibody 66387-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases | ||
PLoS Pathog An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS). | ||
iScience ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1 | ||
Biochem Pharmacol Deficiency of purinergic P2X4 receptor alleviates experimental autoimmune hepatitis in mice | ||
Emerg Microbes Infect Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation. | ||
Mol Med MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription |