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GSDMD Monoclonal antibody, PBS Only

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E6C7

Cat no : 66387-1-PBS

Print datasheet

Synonyms

DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1

验证数据展示

  • WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

    WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

    IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66387-1-Ig(GSDMD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

    IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

    IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.

产品信息

66387-1-PBS targets GSDMD in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen GSDMD fusion protein Ag16827 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name gasdermin D
Synonyms DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight 47-53 kDa
GenBank Accession NumberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_2881763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP57764
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.