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GSDMD Monoclonal antibody, PBS Only

GSDMD Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E6C7

Cat No : 66387-1-PBS

Print datasheet

Synonyms

DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1

验证数据展示

  • WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

    WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

    IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66387-1-Ig(GSDMD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

    IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

    IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

产品信息

66387-1-PBS targets GSDMD in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IF, ELISA Application Description
Tested Reactivity human
Immunogen GSDMD fusion protein Ag16827 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name gasdermin D
Synonyms DF5L, DFNA5L, FKSG10, gasdermin D, GSDMD, GSDMDC1
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight 47-53 kDa
GenBank Accession NumberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP57764
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.