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  • Featured Product
  • KD/KO Validated

GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11051-1-AP

Print datasheet

Synonyms

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

验证数据展示

  • WB analysis using 11051-1-AP

    WB analysis using 11051-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11051-1-AP

    WB analysis using 11051-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11051-1-AP

    IHC staining of human prostate cancer using 11051-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 11051-1-AP

    IHC staining of human prostate cancer using 11051-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 11051-1-AP

    FC experiment of K-562 using 11051-1-AP

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human GSNOR,ADH5 (11051-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of mouse liver using 11051-1-AP

    IP experiment of mouse liver using 11051-1-AP

    IP result of anti-GSNOR,ADH5 (IP:11051-1-AP, 4ug; Detection:11051-1-AP 1:5000) with mouse liver tissue lysate 1840 ug.

  • IF Staining of HepG2 using 11051-1-AP

    IF Staining of HepG2 using 11051-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 11051-1-AP

    IHC staining of human colon using 11051-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11051-1-AP (GSNOR,ADH5 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, mouse kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman prostate cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11051-1-AP targets GSNOR,ADH5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken, zebrafish
Immunogen GSNOR,ADH5 fusion protein Ag1533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Synonyms ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC070491
Gene Symbol ADH5
Gene ID (NCBI) 128
RRIDAB_593422
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

实验方案

Product Specific Protocols
WB protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IHC protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IF protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
IP protocol for GSNOR,ADH5 antibody 11051-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Blood

Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.

Authors - Anfeng Mu
  • KO Validated
View Article
humanWB

Nat Commun

Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells

Authors - Akiko Tomita
View Article
human,mouseWB,IHC

Mol Cell

Two Aldehyde Clearance Systems Are Essential to Prevent Lethal Formaldehyde Accumulation in Mice and Humans.

Authors - Felix A Dingler
  • KO Validated
View Article
mouseWB

Cancer Res

S-nitrosylation of the Mitochondrial Chaperone TRAP1 Sensitizes Hepatocellular Carcinoma Cells to Inhibitors of Succinate Dehydrogenase.

Authors - Salvatore Rizza
  • KO Validated
View Article
mouseWB

Antioxid Redox Signal

GSNOR deficiency enhances in situ skeletal muscle strength, fatigue resistance and RyR1 S-nitrosylation without impacting mitochondrial content and activity.

Authors - Younghye Moon
  • KO Validated
View Article
mouseWB

Redox Biol

GSNOR modulates hyperhomocysteinemia-induced T cell activation and atherosclerosis by switching Akt S-nitrosylation to phosphorylation.

Authors - Jing Li
  • KD Validated
View Article