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验证数据展示

  • FC experiment of K-562 using CL488-11051

    FC experiment of K-562 using CL488-11051

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GSNOR,ADH5 (CL488-11051) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-11051

    IF Staining of HepG2 using CL488-11051

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GSNOR,ADH5 antibody (CL488-11051) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-11051

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

ADH5, ADH 3, ADH 5, ADH3, ADHX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 11051-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11051 targets GSNOR,ADH5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 GSNOR,ADH5 fusion protein Ag1533 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alcohol dehydrogenase 5 (class III), chi polypeptide
别名 ADH5, ADH 3, ADH 5, ADH3, ADHX
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC070491
基因名称 ADH5
Gene ID (NCBI) 128
RRIDAB_3083891
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11766
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GSNOR,ADH5 antibody CL488-11051Download protocol
Standard Protocols
Click here to view our Standard Protocols