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GSR Recombinant antibody

GSR Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241934D2

Cat no : 84480-2-RR

Print datasheet

Synonyms

GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7

验证数据展示

  • WB analysis using 84480-2-RR

    WB analysis using 84480-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84480-2-RR (GSR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84480-2-RR

    IF Staining of HeLa using 84480-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSR antibody (84480-2-RR, Clone: 241934D2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84480-2-RR

    Affinity and Kinetic Characterization of 84480-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84480-2-RR against Human GSR were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, HeLa cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84480-2-RR targets GSR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GSR fusion protein Ag13080 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glutathione reductase
Synonyms GRD1, GRase, glutathione reductase, GLUR, EC:1.8.1.7
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC069244
Gene Symbol GSR
Gene ID (NCBI) 2936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00390
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSR(Glutathione reductase) is also named as GLUR, GRD1 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.It was first found in a black American a variant red cell GSR characterized by greater electrophoretic mobility and enzyme activity per unit of hemoglobin than the normal.This protein maintains high levels of reduced glutathione in the cytosol.It has 5 isoforms produced by alternative splicing and alternative initiation.

实验方案

Product Specific Protocols
WB protocol for GSR antibody 84480-2-RRDownload protocol
IF protocol for GSR antibody 84480-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols