验证数据展示
经过测试的应用
Positive WB detected in | CHO cells, rat liver tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
67598-1-Ig targets GSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, hamster samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | Human, Mouse, Rat, pig, hamster |
文献引用反应性 | human |
免疫原 | GSS fusion protein Ag8354 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | glutathione synthetase |
别名 | Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS |
计算分子量 | 474 aa, 52 kDa |
观测分子量 | 52 kDa |
GenBank蛋白编号 | BC007927 |
基因名称 | GSS |
Gene ID (NCBI) | 2937 |
RRID | AB_2882804 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P48637 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for GSS antibody 67598-1-Ig | Download protocol |
IHC protocol for GSS antibody 67598-1-Ig | Download protocol |
IF protocol for GSS antibody 67598-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
World J Gastroenterol Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells | ||
J Biol Chem Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity |