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GSS Monoclonal antibody
GSS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, pig, hamster and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1F12G1
验证数据展示
经过测试的应用
| Positive WB detected in | CHO cells, rat liver tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GSS Ready-To-Use IHC Kit. GSS primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67598-1-Ig targets GSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, hamster samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Mouse, Rat, pig, hamster |
| Cited Reactivity | human |
| Immunogen | GSS fusion protein Ag8354 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glutathione synthetase |
| Synonyms | Glutathione synthase, glutathione synthetase, GSH S, GSH synthetase, GSHS, GSS |
| Calculated Molecular Weight | 474 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC007927 |
| Gene Symbol | GSS |
| Gene ID (NCBI) | 2937 |
| RRID | AB_2882804 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P48637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GSS antibody 67598-1-Ig | Download protocol |
| IHC protocol for GSS antibody 67598-1-Ig | Download protocol |
| IF protocol for GSS antibody 67598-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
World J Gastroenterol Bile acids inhibit ferroptosis sensitivity through activating farnesoid X receptor in gastric cancer cells | ||
J Biol Chem Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity |