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GSTT1 Monoclonal antibody, PBS Only

GSTT1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A4C11

Cat No : 67645-1-PBS

Print datasheet

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1

验证数据展示

  • IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTT1 antibody included
  • WB analysis using 67645-1-Ig (same clone as 67645-1-PBS)

    WB analysis using 67645-1-Ig (same clone as 67645-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IHC staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSTT1 antibody included
  • IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    IF Staining of mouse liver using 67645-1-Ig (same clone as 67645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67645-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GSTT1 Ready-To-Use IHC Kit. GSTT1 primary antibody included.

产品信息

67645-1-PBS targets GSTT1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, pig, mouse
Immunogen GSTT1 fusion protein Ag8573 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase theta 1
Synonyms Glutathione transferase T1 1, GST class theta 1, GSTT1
Calculated Molecular Weight 240 aa, 27 kDa
Observed Molecular Weight 28 kDa, 30 kDa
GenBank Accession NumberBC007065
Gene Symbol GSTT1
Gene ID (NCBI) 2952
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30711
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.