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GSTT1 Monoclonal antibody

GSTT1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A4C11

Cat no : 67645-1-Ig

Print datasheet

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1

验证数据展示

  • IHC staining of mouse liver using 67645-1-Ig

    IHC staining of mouse liver using 67645-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSTT1 antibody included
  • WB analysis using 67645-1-Ig

    WB analysis using 67645-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67645-1-Ig (GSTT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 67645-1-Ig

    IHC staining of mouse liver using 67645-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67645-1-Ig (GSTT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GSTT1 antibody included
  • IF Staining of mouse liver using 67645-1-Ig

    IF Staining of mouse liver using 67645-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 67645-1-Ig

    IF Staining of mouse liver using 67645-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using GSTT1 antibody (67645-1-Ig, Clone: 1A4C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inpig lung tissue, pig liver tissue, K-562 cells, rat liver tissue, mouse brain tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy GSTT1 Ready-To-Use IHC Kit. GSTT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67645-1-Ig targets GSTT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, pig, mouse
Immunogen GSTT1 fusion protein Ag8573 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase theta 1
Synonyms Glutathione transferase T1 1, GST class theta 1, GSTT1
Calculated Molecular Weight 240 aa, 27 kDa
Observed Molecular Weight 28 kDa, 30 kDa
GenBank Accession NumberBC007065
Gene Symbol GSTT1
Gene ID (NCBI) 2952
RRIDAB_2882845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30711
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

实验方案

Product Specific Protocols
WB protocol for GSTT1 antibody 67645-1-IgDownload protocol
IHC protocol for GSTT1 antibody 67645-1-IgDownload protocol
IF protocol for GSTT1 antibody 67645-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols