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验证数据展示

  • IF Staining of mouse liver using CL488-67645

    IF Staining of mouse liver using CL488-67645

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 GSTT1 antibody (CL488-67645, Clone: 1A4C11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated GSTT1 Monoclonal antibody

GSTT1 Monoclonal Antibody for IF-P
Cat No. CL488-67645

Print datasheet

CloneNo. 1A4C11

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF-P

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy GSTT1 Ready-To-Use IHC Kit. GSTT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67645-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67645 targets GSTT1 in IF-P applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, mouse
Immunogen GSTT1 fusion protein Ag8573 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutathione S-transferase theta 1
Synonyms Glutathione transferase T1 1, GST class theta 1, GSTT1
Calculated Molecular Weight 240 aa, 27 kDa
Observed Molecular Weight 28 kDa, 30 kDa
GenBank Accession NumberBC007065
Gene Symbol GSTT1
Gene ID (NCBI) 2952
RRIDAB_2934556
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30711
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GSTT1 antibody CL488-67645Download protocol
Standard Protocols
Click here to view our Standard Protocols