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验证数据展示

  • WB analysis of Caco-2 using 67782-1-Ig

    WB analysis of Caco-2 using 67782-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GUCY2C Monoclonal antibody

GUCY2C Monoclonal Antibody for WB, ELISA
Cat No. 67782-1-Ig

产品说明书

CloneNo. 1F5F6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

GC C, Guanylyl cyclase C, GUC2C, GUCY2C, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaco-2 cells, HaCaT cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67782-1-Ig targets GUCY2C in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 GUCY2C fusion protein Ag19256 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 guanylate cyclase 2C (heat stable enterotoxin receptor)
别名 GC C, Guanylyl cyclase C, GUC2C, GUCY2C, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR
计算分子量 1073 aa, 123 kDa
观测分子量130 kDa
GenBank蛋白编号BC136544
基因名称 GUCY2C
Gene ID (NCBI) 2984
RRIDAB_2918546
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP25092
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GUCY2C is also named as GUC2C, STAR(Heat-stable enterotoxin receptor), Guanylyl cyclase C and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a receptor for the E.coli heat-stable enterotoxin and considered to be the true apical membrane receptor for the endogenous ligands uroguanylin and guanylin. GUCY2C is not present in the endocrine pancreas, but is localized exclusively to the ductal system which fully corresponds to the GUCY2C activity detected in the exocrine ductal system, but particularly not in islets of Langerhans. GUCY2C with a molecular mass of 130 kDa is detected in pancreatic and intestinal extracts.(PMID:11431160). The full length protein is 123 kDa and has a signal peptide and nine glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for GUCY2C antibody 67782-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols