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GUCY2C Monoclonal antibody

GUCY2C Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5F6

Cat no : 67782-1-Ig

Print datasheet

Synonyms

GC C, Guanylyl cyclase C, GUC2C, GUCY2C, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR

验证数据展示

  • WB analysis of Caco-2 using 67782-1-Ig

    WB analysis of Caco-2 using 67782-1-Ig

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67782-1-Ig (GUCY2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCaco-2 cells, HaCaT cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67782-1-Ig targets GUCY2C in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen GUCY2C fusion protein Ag19256 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanylate cyclase 2C (heat stable enterotoxin receptor)
Synonyms GC C, Guanylyl cyclase C, GUC2C, GUCY2C, hSTAR, Intestinal guanylate cyclase, STA receptor, STAR
Calculated Molecular Weight 1073 aa, 123 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC136544
Gene Symbol GUCY2C
Gene ID (NCBI) 2984
RRIDAB_2918546
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25092
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GUCY2C is also named as GUC2C, STAR(Heat-stable enterotoxin receptor), Guanylyl cyclase C and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It is a receptor for the E.coli heat-stable enterotoxin and considered to be the true apical membrane receptor for the endogenous ligands uroguanylin and guanylin. GUCY2C is not present in the endocrine pancreas, but is localized exclusively to the ductal system which fully corresponds to the GUCY2C activity detected in the exocrine ductal system, but particularly not in islets of Langerhans. GUCY2C with a molecular mass of 130 kDa is detected in pancreatic and intestinal extracts.(PMID:11431160). The full length protein is 123 kDa and has a signal peptide and nine glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for GUCY2C antibody 67782-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols