验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
55127-1-AP targets GUCY2D in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanylate cyclase 2D, membrane (retina-specific) |
| 别名 | CORD5, CORD6, CYGD, Guanylate cyclase 2D, retinal, GUC1A4, GUC2D, GUCY2D, LCA, LCA1, retGC, RETGC 1, RETGC1, Retinal guanylyl cyclase 1, ROS GC, ROS GC1 |
| 计算分子量 | 120 kDa |
| 观测分子量 | 120 kDa, 95 kDa |
| GenBank蛋白编号 | NM_000180 |
| 基因名称 | GUCY2D |
| Gene ID (NCBI) | 3000 |
| RRID | AB_10804281 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q02846 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GUCY2D, also named as CORD6, GUC1A4, GUC2D, RETGC, RETGC1 and ROS-GC, belongs to the adenylyl cyclase class-4/guanylyl cyclase family. It probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. A number of nonsense and frameshift mutations in the GUCY2D gene have been identified in LCA1 patients. Defects in GUCY2D are the cause of cone-rod dystrophy type 6 (CORD6). GUCY2D is responsible for many reported cases of autosomal dominant CRDs. The antibody is specific to GUCY2D.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GUCY2D antibody 55127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation. | ||
J Biol Chem The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. |
