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验证数据展示

  • WB analysis of HepG2 using 17489-1-AP

    WB analysis of HepG2 using 17489-1-AP

    WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells.

  • WB analysis using 17489-1-AP

    WB analysis using 17489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus using 17489-1-AP

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human oesophagus using 17489-1-AP

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 17489-1-AP

    IF Staining of HepG2 using 17489-1-AP

    Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 17489-1-AP

    IP experiment of HeLa using 17489-1-AP

    IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Featured Product
  • KD/KO Validated

GUF1 Polyclonal antibody

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17489-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, mouse pancreas tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus tissue, human lung cancer tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17489-1-AP targets GUF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GUF1 fusion protein Ag11579 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GUF1 GTPase homolog (S. cerevisiae)
Synonyms Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4
Calculated Molecular Weight 669 aa, 74 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC036768
Gene Symbol GUF1
Gene ID (NCBI) 60558
RRIDAB_2115960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N442
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

实验方案

Product Specific Protocols
WB protocol for GUF1 antibody 17489-1-APDownload protocol
IHC protocol for GUF1 antibody 17489-1-APDownload protocol
IF protocol for GUF1 antibody 17489-1-APDownload protocol
IP protocol for GUF1 antibody 17489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols