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验证数据展示

  • WB analysis of HepG2 using 17489-1-AP

    WB analysis of HepG2 using 17489-1-AP

    WB result of GUF1 antibody (17489-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GUF1 transfected HepG2 cells.

  • WB analysis using 17489-1-AP

    WB analysis using 17489-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17489-1-AP (GUF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus using 17489-1-AP

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human oesophagus using 17489-1-AP

    IHC staining of human oesophagus using 17489-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 17489-1-AP (GUF1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 17489-1-AP

    IF Staining of HepG2 using 17489-1-AP

    Immunofluorescent analysis of HepG2 cells, using GUF1 antibody 17489-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 17489-1-AP

    IP experiment of HeLa using 17489-1-AP

    IP result of anti-GUF1 (IP:17489-1-AP, 3ug; Detection:17489-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Featured Product
  • KD/KO验证

GUF1 Polyclonal antibody

GUF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17489-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells, mouse pancreas tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus tissue, human lung cancer tissue, human kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17489-1-AP targets GUF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 GUF1 fusion protein Ag11579 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GUF1 GTPase homolog (S. cerevisiae)
别名 Translation factor GUF1, mitochondrial, Ribosomal back-translocase, GTPase GUF1, Elongation factor 4 homolog, EF-4
计算分子量 669 aa, 74 kDa
观测分子量 70 kDa
GenBank蛋白编号BC036768
基因名称 GUF1
Gene ID (NCBI) 60558
RRIDAB_2115960
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N442
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

实验方案

Product Specific Protocols
WB protocol for GUF1 antibody 17489-1-APDownload protocol
IHC protocol for GUF1 antibody 17489-1-APDownload protocol
IF protocol for GUF1 antibody 17489-1-APDownload protocol
IP protocol for GUF1 antibody 17489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols