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验证数据展示

  • IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

    IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-3-RR (GYPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

    IHC staining of human placenta using 85182-3-RR (same clone as 85182-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85182-3-RR (GYPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85182-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Glycophorin A antibody included
  • Cytometric bead array standard curve of MP01888-1

    Cytometric bead array standard curve of MP01888-1

    Cytometric bead array standard curve of MP01888-1, Glycophorin A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85182-2-PBS. Detection antibody: 85182-3-PBS. Standard: Eg2336. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01888-2

    Cytometric bead array standard curve of MP01888-2

    Cytometric bead array standard curve of MP01888-2, Glycophorin A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85182-1-PBS. Detection antibody: 85182-3-PBS. Standard: Eg2336. Range: 0.313-40 ng/mL.

Glycophorin A/CD235a Recombinant antibody, PBS Only (Detector)

Glycophorin A/CD235a Uni-rAbTM Recombinant Antibody for IHC, Cytometric bead array, Indirect ELISA
Cat No. 85182-3-PBS

产品说明书

CloneNo. 242822H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, Cytometric bead array, Indirect ELISA

别名

Glycophorin A, GPErik, GPA, Glycophorin-A, CD235a

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.

产品信息

85182-3-PBS targets Glycophorin A/CD235a as part of a matched antibody pair:

MP01888-1: 85182-2-PBS capture and 85182-3-PBS detection (validated in Cytometric bead array)

MP01888-2: 85182-1-PBS capture and 85182-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycophorin A (MNS blood group)
别名 Glycophorin A, GPErik, GPA, Glycophorin-A, CD235a
计算分子量 150 aa, 16 kDa
GenBank蛋白编号BC005319
基因名称 Glycophorin A
Gene ID (NCBI) 2993
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02724
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycophorin A (GYPA), also known as CD235a, is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). Glycophorin A represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)