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CoraLite® Plus 488-conjugated Galectin-1 Monoclonal antibody

Galectin-1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, IF-P, FC (Intra) and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G10D2

Cat no : CL488-60223

Print datasheet

Synonyms

Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein



经过测试的应用

Positive IF-P detected inhuman colon cancer tissue, U2OS cells
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy LGALS1 Ready-To-Use IHC Kit. LGALS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60223-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-60223 targets Galectin-1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen Galectin-1 fusion protein Ag2432 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 1
Synonyms Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC020675
Gene Symbol Galectin-1
Gene ID (NCBI) 3956
RRIDAB_2919238
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09382
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Galectin-1 antibody CL488-60223Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Sci Rep

The role of GCNT1 mediated O-glycosylation in aggressive prostate cancer

Authors - Kirsty Hodgson

mBio

Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection

Authors - Guorong Sun