验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue, U2OS cells |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy LGALS1 Ready-To-Use IHC Kit. LGALS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60223-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-60223 targets Galectin-1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | Galectin-1 fusion protein Ag2432 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lectin, galactoside-binding, soluble, 1 |
| 别名 | Gal 1, Beta-galactoside-binding lectin L-14-I, 3G10D2, 14 kDa lectin, 14 kDa laminin-binding protein |
| 计算分子量 | 15 kDa |
| 观测分子量 | 13 kDa |
| GenBank蛋白编号 | BC020675 |
| 基因名称 | Galectin-1 |
| Gene ID (NCBI) | 3956 |
| RRID | AB_2919238 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P09382 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Galectin-1 antibody CL488-60223 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
mBio Herpes simplex virus type 1 modifies the protein composition of extracellular vesicles to promote neurite outgrowth and neuroinfection |