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验证数据展示

  • IF Staining of human colon cancer using CL594-66686

    IF Staining of human colon cancer using CL594-66686

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-4 antibody (CL594-66686, Clone: 3C2G3 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL594-66686

    IF Staining of human colon cancer using CL594-66686

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 Galectin-4 antibody (CL594-66686, Clone: 3C2G3 ) at dilution of 1:200.

CoraLite®594-conjugated Galectin-4 Monoclonal antibody

Galectin-4 Monoclonal Antibody for IF-P
Cat No. CL594-66686

产品说明书

CloneNo. 3C2G3

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF-P

别名

Antigen NY CO 27, Gal 4, GAL4, Galectin 4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy LGALS4 Ready-To-Use IHC Kit. LGALS4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66686-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66686 targets Galectin-4 in IF-P applications and shows reactivity with Human samples.

经测试应用 IF-P Application Description
经测试反应性 Human
免疫原 Galectin-4 fusion protein Ag4049 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 4
别名 Antigen NY CO 27, Gal 4, GAL4, Galectin 4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4
计算分子量 323 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC034750
基因名称 Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2920023
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP56470
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

实验方案

Product Specific Protocols
IF protocol for CL594 Galectin-4 antibody CL594-66686Download protocol
Standard Protocols
Click here to view our Standard Protocols