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验证数据展示

  • WB analysis using CL488-66445

    WB analysis using CL488-66445

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using CL488-66445

    WB analysis using CL488-66445

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated Gamma Catenin Monoclonal antibody

Gamma Catenin Monoclonal Antibody for WB
Cat No. CL488-66445

Print datasheet

CloneNo. 1A5D10

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, Canine

Applications

WB

Synonyms

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, T-47D cells
Planning an IHC experiment? We recommend our IHCeasy JUP Ready-To-Use IHC Kit. JUP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66445-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66445 targets Gamma Catenin in WB applications and shows reactivity with human, rat, pig, Canine samples.

Tested Applications WB Application Description
Tested Reactivity human, rat, pig, Canine
Immunogen Gamma Catenin fusion protein Ag1627 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name junction plakoglobin
Synonyms ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin
Calculated Molecular Weight 82 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC011865
Gene Symbol Gamma Catenin
Gene ID (NCBI) 3728
RRIDAB_3084247
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14923
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Gamma Catenin antibody CL488-66445Download protocol
Standard Protocols
Click here to view our Standard Protocols