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验证数据展示

  • WB analysis using CL488-66445

    WB analysis using CL488-66445

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using CL488-66445

    WB analysis using CL488-66445

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66445 (Gamma Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated Gamma Catenin Monoclonal antibody

Gamma Catenin Monoclonal Antibody for WB
Cat No. CL488-66445

产品说明书

CloneNo. 1A5D10

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig, Canine

应用

WB

别名

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, T-47D cells
Planning an IHC experiment? We recommend our IHCeasy JUP Ready-To-Use IHC Kit. JUP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66445-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66445 targets Gamma Catenin in WB applications and shows reactivity with human, rat, pig, Canine samples.

经测试应用 WB Application Description
经测试反应性 human, rat, pig, Canine
免疫原 Gamma Catenin fusion protein Ag1627 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 junction plakoglobin
别名 ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin
计算分子量 82 kDa
观测分子量 82 kDa
GenBank蛋白编号BC011865
基因名称 Gamma Catenin
Gene ID (NCBI) 3728
RRIDAB_3084247
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP14923
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Gamma Catenin antibody CL488-66445Download protocol
Standard Protocols
Click here to view our Standard Protocols