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验证数据展示

  • WB analysis of Jurkat using 84199-2-RR

    WB analysis of Jurkat using 84199-2-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84199-2-RR (GMNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 84199-2-RR

    WB analysis of HEK-293 using 84199-2-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 84199-2-RR (GMNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84199-2-RR

    IHC staining of human colon cancer using 84199-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84199-2-RR (Geminin antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 84199-2-RR

    IF Staining of HepG2 using 84199-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Geminin antibody (84199-2-RR, Clone: 240835B5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84199-2-RR

    IF Staining of HeLa using 84199-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Geminin antibody (84199-2-RR, Clone: 240835B5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84199-2-RR

    Affinity and Kinetic Characterization of 84199-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84199-2-RR against Human Geminin were performed. The affinity constant is below 1 pM.

Geminin Recombinant antibody

Geminin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84199-2-RR

Print datasheet

CloneNo. 240835B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GMNN, MGORS6, Gem, 240835B5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84199-2-RR targets Geminin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Geminin fusion protein Ag1076 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name geminin, DNA replication inhibitor
Synonyms GMNN, MGORS6, Gem, 240835B5
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 28-29 kDa
GenBank Accession NumberBC005185
Gene Symbol GMNN
Gene ID (NCBI) 51053
RRIDAB_3671755
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO75496
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)

实验方案

Product Specific Protocols
WB protocol for Geminin antibody 84199-2-RRDownload protocol
IF protocol for Geminin antibody 84199-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols