CoraLite® Plus 488-conjugated Geminin Recombinant antibody

Geminin Recombinant Antibody for IF/ICC
Cat No. CL488-84199-2

产品说明书

CloneNo. 240835B5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

GMNN, MGORS6, Gem, 240835B5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 84199-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84199-2 targets Geminin in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Geminin fusion protein Ag1076 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 geminin, DNA replication inhibitor
别名 GMNN, MGORS6, Gem, 240835B5
计算分子量 24 kDa
观测分子量 28-29 kDa
GenBank蛋白编号BC005185
基因名称 GMNN
Gene ID (NCBI) 51053
RRIDAB_3673347
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75496
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Geminin antibody CL488-84199-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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