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Glucocorticoid receptor Recombinant antibody, PBS Only

Glucocorticoid receptor Recombinant Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2F22

Cat No : 82619-4-PBS

Print datasheet

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

验证数据展示

  • WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

    WB analysis using 82619-4-RR (same clone as 82619-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IP experiment of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IP result of anti-Glucocorticoid receptor (IP:82619-4-RR, 4ug; Detection:82619-4-RR 1:8000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    IHC staining of human prostate cancer using 82619-4-RR (same clone as 82619-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    IF Staining of HepG2 using 82619-4-RR (same clone as 82619-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (82619-4-RR, Clone: 2F22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82619-4-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.

产品信息

82619-4-PBS targets Glucocorticoid receptor in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse
Immunogen Glucocorticoid receptor fusion protein Ag21146 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Synonyms GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 94-97 kDa
GenBank Accession NumberBC015610
Gene Symbol NR3C1
Gene ID (NCBI) 2908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04150
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).