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验证数据展示

  • WB analysis using 82619-4-RR

    WB analysis using 82619-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 82619-4-RR

    IP experiment of HepG2 using 82619-4-RR

    IP result of anti-Glucocorticoid receptor (IP:82619-4-RR, 4ug; Detection:82619-4-RR 1:8000) with HepG2 cells lysate 1920 ug.

  • IHC staining of human prostate cancer using 82619-4-RR

    IHC staining of human prostate cancer using 82619-4-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IHC staining of human prostate cancer using 82619-4-RR

    IHC staining of human prostate cancer using 82619-4-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82619-4-RR (Glucocorticoid receptor antibody) at dilution of 1:350 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IF Staining of HepG2 using 82619-4-RR

    IF Staining of HepG2 using 82619-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (82619-4-RR, Clone: 2F22 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82619-4-RR

    Affinity and Kinetic Characterization of 82619-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82619-4-RR against Human Glucocorticoid receptor were performed. The affinity constant is 0.693 nM.

Glucocorticoid receptor Recombinant antibody

Glucocorticoid receptor Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 82619-4-RR

产品说明书

CloneNo. 2F22

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

NR3C1, GRL, GR, GCR, GCCR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, U-251 cells, DU 145 cells, MCF-7 cells, L-929 cells, RAW 264.7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:175-1:700
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82619-4-RR targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 Glucocorticoid receptor fusion protein Ag21146 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
别名 NR3C1, GRL, GR, GCR, GCCR
计算分子量 86 kDa
观测分子量 94-97 kDa
GenBank蛋白编号BC015610
基因名称 Glucocorticoid receptor
Gene ID (NCBI) 2908
RRIDAB_3086492
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04150
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

实验方案

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 82619-4-RRDownload protocol
IHC protocol for Glucocorticoid receptor antibody 82619-4-RRDownload protocol
IF protocol for Glucocorticoid receptor antibody 82619-4-RRDownload protocol
IP protocol for Glucocorticoid receptor antibody 82619-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols