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Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for WB, IF-P, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF-P, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6G4

Cat No : 66323-2-Ig

Print datasheet

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

验证数据展示

  • WB analysis of Jurkat using 66323-2-Ig

    WB analysis of Jurkat using 66323-2-Ig

    WB result of Glutamine synthetase antibody (66323-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.

  • IHC staining of human liver cancer using 66323-2-Ig

    IHC staining of human liver cancer using 66323-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLUL antibody included
  • IHC staining of human liver cancer using 66323-2-Ig

    IHC staining of human liver cancer using 66323-2-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-2-Ig (Glutamine Synthetase antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GLUL antibody included
  • IF Staining of mouse liver using 66323-2-Ig

    IF Staining of mouse liver using 66323-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (66323-2-Ig, Clone: 1B6G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue, rat liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

66323-2-Ig targets Glutamine Synthetase in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF-P, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human, mouse, rat
Cited Reactivitycanine
Immunogen Glutamine Synthetase fusion protein Ag6309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamate-ammonia ligase (glutamine synthetase)
Synonyms GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC011700
Gene Symbol GLUL
Gene ID (NCBI) 2752
RRIDAB_2881704
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15104
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Glutamine Synthetase antibody 66323-2-IgDownload protocol
IHC protocol for Glutamine Synthetase antibody 66323-2-IgDownload protocol
IF protocol for Glutamine Synthetase antibody 66323-2-IgDownload protocol
FC protocol for Glutamine Synthetase antibody 66323-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineIF

Neural Regen Res

Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma

Authors - Yu Huan
View Article