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验证数据展示

  • IF Staining of mouse brain using CL488-66323-2

    IF Staining of mouse brain using CL488-66323-2

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 Glutamine Synthetase antibody (CL488-66323-2, Clone: 1B6G4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

CoraLite® Plus 488-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for IF-P
Cat No. CL488-66323-2

产品说明书

CloneNo. 1B6G4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF-P

别名

GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy GLUL Ready-To-Use IHC Kit. GLUL primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66323-2-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66323-2 targets Glutamine Synthetase in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Glutamine Synthetase fusion protein Ag6309 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamate-ammonia ligase (glutamine synthetase)
别名 GLUL, 1B6G4, EC:2.3.1.225, EC:6.3.1.2, GLNS
计算分子量 374 aa, 42 kDa
观测分子量42 kDa
GenBank蛋白编号BC011700
基因名称 Glutamine Synthetase
Gene ID (NCBI) 2752
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP15104
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Glutamine Synthetase antibody CL488-66323-2Download protocol
Standard Protocols
Click here to view our Standard Protocols