验证数据展示
经过测试的应用
Positive FC (Intra) detected in | SH-SY5Y cells, HeLa cells |
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83241-1-RR targets Glypican 2 in ELISA applications and shows reactivity with human samples.
Tested Applications | ELISA Application Description |
Tested Reactivity | human |
Immunogen | Glypican 2 fusion protein Ag4041 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Affinity | KD<1.00 x 10-12M KOff<1.00 x 10-6M KOn=1.07 x 105M |
Full Name | glypican 2 |
Synonyms | GPC2, Secreted glypican-2, FLJ38962, 230518A4 |
Calculated Molecular Weight | 579 aa, 63 kDa |
Observed Molecular Weight | 62,70-100 kDa |
GenBank Accession Number | BC027972 |
Gene Symbol | GPC2 |
Gene ID (NCBI) | 221914 |
RRID | AB_3670917 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8N158 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).
实验方案
Product Specific Protocols | |
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FC protocol for Glypican 2 antibody 83241-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |