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Anti-Mouse Gm-csf Rabbit Recombinant Antibody

Gm-csf Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230286E11

Cat no : 98014-1-RR

Print datasheet

Synonyms

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

验证数据展示

  • FC experiment of C57BL/6 CD3 T cells using 98014-1-RR

    FC experiment of C57BL/6 CD3 T cells using 98014-1-RR

    1x10^6 unstimulated or PMA and ionomycin in presence of protein transport inhibitors stimulated C57BL/6 CD3 T cells were intracellularly stained with 0.25 ug Anti-Mouse Gm-csf (98014-1-RR, Clone: 230286E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.5 ug FITC Plus Anti-Mouse CD3 (17A2) (FITC-65077, Clone: 17A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98014-1-RR

    Affinity and Kinetic Characterization of 98014-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98014-1-RR against Human Gm-csf were performed. The affinity constant is 2.25 nM.

经过测试的应用

Positive FC (Intra) detected inPMA and ionomycin stimulated C57BL/6 CD3 T cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98014-1-RR targets Gm-csf in FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Synonyms Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2
GenBank Accession NumberNM-009969
Gene Symbol GM-CSF
Gene ID (NCBI) 12981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01587
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.

实验方案

Product Specific Protocols
FC protocol for Gm-csf antibody 98014-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols