验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PMA and ionomycin stimulated C57BL/6 CD3 T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98014 targets GM-CSF in FC (Intra) applications and shows reactivity with mouse samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | colony stimulating factor 2 (granulocyte-macrophage) |
| 别名 | Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2 |
| GenBank蛋白编号 | NM-009969 |
| 基因名称 | GM-CSF |
| Gene ID (NCBI) | 12981 |
| RRID | AB_3673657 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01587 |
| 储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 GM-CSF antibody CL647-98014 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |