验证数据展示
经过测试的应用
| Positive WB detected in | NK-92 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84583-4-RR targets Granzyme A in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Granzyme A fusion protein Ag1818 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) |
| 别名 | GrA, GZMA, Fragmentin 1, EC:3.4.21.78, Cytotoxic T-lymphocyte proteinase 1 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC015739 |
| 基因名称 | Granzyme A |
| Gene ID (NCBI) | 3001 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P12544 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Granzyme A antibody 84583-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |