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Granzyme B Recombinant antibody, PBS Only

Granzyme B Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240761C6

Cat no : 83763-5-PBS

Print datasheet

Synonyms

GZMB, CSP B, CGL1, CGL 1, CCPI

验证数据展示

  • WB analysis of NK-92 using 83763-5-RR (same clone as 83763-5-PBS)

    WB analysis of NK-92 using 83763-5-RR (same clone as 83763-5-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 83763-5-RR (GZMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83763-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83763-5-RR

    Affinity and Kinetic Characterization of 83763-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83763-5-RR against Human Granzyme B were performed. The affinity constant is 0.281 nM.

产品信息

The immunogen of 83763-5-PBS is Granzyme B Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Synonyms GZMB, CSP B, CGL1, CGL 1, CCPI
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC030195
Gene Symbol Granzyme B
Gene ID (NCBI) 3002
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10144
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.