Granzyme B Recombinant antibody
Granzyme B Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240761C6
验证数据展示
经过测试的应用
Positive WB detected in | NK-92 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83763-5-RR targets Granzyme B in WB, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Fusion Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
Synonyms | GZMB, CSP B, CGL1, CGL 1, CCPI |
Calculated Molecular Weight | 247 aa, 28 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC030195 |
Gene Symbol | Granzyme B |
Gene ID (NCBI) | 3002 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P10144 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Granzyme B antibody 83763-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |